rabbit anti irf8  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Integrative Multi-Omics Analysis Reveals Molecular Signatures of Recurrence in Paired Primary and Recurrent High-Grade Serous Ovarian Cancer

doi: 10.3390/ijms27020948

Figure Lengend Snippet: Differential expression and prognostic significance of candidate differentially expressed genes (DEGs) in primary and recurrent high-grade serous ovarian cancer. ( A ) Heatmap showing the expression levels of selected DEGs in primary and recurrent tumor samples. Columns represent individual tumor samples (primary, yellow; recurrent, green), and rows represent DEGs. Color intensity indicates relative expression levels, ranging from blue (low) to red (high). ( B ) Kaplan–Meier curves showing time to recurrence for patients stratified into high- and low-expression groups for each indicated DEG ( GPR68 , IL7R , IRF8 , PTPRC , WDFY4 , C15orf39 , NSG1 ). Statistical significance was evaluated using the log-rank test, with p -values shown on each plot.

Article Snippet: The primary antibodies used were IL7R (Abcam, Cambridge, UK, #ab259806), IRF8 (Cell Signaling, Danvers, MA, USA, #83413), GPR68 (Abcam, #ab61420), PTPRC (Cell Signaling, #13917), WDFY4 (Abcam, #ab122661), and NSG1 (Invitrogen, #PA5-36497).

Techniques: Quantitative Proteomics, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Integrative Multi-Omics Analysis Reveals Molecular Signatures of Recurrence in Paired Primary and Recurrent High-Grade Serous Ovarian Cancer

doi: 10.3390/ijms27020948

Figure Lengend Snippet: Differential protein expression of six candidate genes in matched primary and recurrent high-grade serous ovarian cancer tissues assessed by immunohistochemistry. ( A ) Representative immunohistochemical staining of IL7R, IRF8, GPR68, PTPRC, WDFY4, and NSG1 in tissue microarrays derived from paired primary and recurrent tumor samples. Scale bars represent 50 or 100 µm. Notable differences in expression were observed for IL7R, PTPRC, and NSG1 between matched tumor pairs. ( B ) Paired dot plots showing immunohistochemical staining scores for each of the six proteins. Black lines connect matched tumor pairs (primary and recurrent) from the same patient. Statistical significance of expression differences between primary and recurrent tumor samples was assessed using paired t -tests.

Article Snippet: The primary antibodies used were IL7R (Abcam, Cambridge, UK, #ab259806), IRF8 (Cell Signaling, Danvers, MA, USA, #83413), GPR68 (Abcam, #ab61420), PTPRC (Cell Signaling, #13917), WDFY4 (Abcam, #ab122661), and NSG1 (Invitrogen, #PA5-36497).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining, Derivative Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: HLA-DQB1-mediated B cell-epithelial crosstalk drives EBV-associated inflammation in Hunner-type interstitial cystitis

doi: 10.1016/j.omtn.2025.102783

Figure Lengend Snippet: SCENIC analysis of transcriptional regulators in the HIC microenvironment (A) Boxplots illustrating the number of cells per regulon (left) and the number of active regulons per cell (right). (B) Bubble plot of the top regulons identified through SCENIC, showing their cell-type specificity and relative activity scores. (C) Heatmap displaying the transcriptional activity of key regulons across different cell populations in HIC samples. (D) Scatterplot ranking transcription factors based on their regulon specificity score (RSS) in B cells. (E) Protein expression of IRF8 in bladder cancer cells (RT4 and HT1376) and LCLs.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in 0.1% PBS with Tween 20 and then probed overnight at 4°C with anti-IRF8 rabbit monoclonal antibody (E6J8Q, Cell Signaling, catalog no. 83413; 1:1,000) followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch; 1:5,000, 1.5 h, room temperature).

Techniques: Activity Assay, Expressing